اثر عصاره متانولی Otostegia persica بر سطح سرمی‌گلوکز و آنزیم‌‌های شاخص عملکرد کبد در موش‌های صحرایی نر دیابتی شده با استرپتوزوتوسین

  The Effect of Methanolic Extract of Otostegia persica on Serum Levels of Glucose and Liver Function Enzymes in Streptozotocin -Induced Diabetic Male Rats     M. Hedayati [1] , I. Pouraboli [2] , M. Mirtajaddini [3]     Received: 15/06/10 Sent for Revision: 04/09/10 Received Revised Manuscript: 17/10/10 Accepted: 31/10/10     Background and Objectives : With respect to antioxidant effects of Otostegia persica and the role of antioxidant agents in the treatment diabetes mellitus, in this study the effect of methanolic extract of Otostegia persica on the serum levels of glucose and liver function enzymes was investigated in diabetic male rats.   Materials and Methods : In the experimented study, type-I diabetes was induced in male rats by the injection of 70 mg/kg, i.p of streptozotocin. Blood samples were collected from rats for measuring glucose, ALT, AST and ALP in two ocassions 1- before inducing diabetes, 2- five days after streptozotocin injection. Diabetes was confirmed in rats if FBS level was above 250 mg/dL. Diabetic rats were divided into 10 groups received 100, 200 and 300 mg/kg extract, glibenclamide (600 µ g/kg) and distilled water (0.5 mL) daily for 6 and 14 days individually by gavage. After 6 and 14 days rats were sacrificed and serum levels of above factors were measured by spectrophotometery.   Results: . Administration of all doses of O. persica extract daily for 6 and 14 days decreased significantly the FBS serum level, significantly (p<0.05) . Administration of extract for 6 days, at doses 100 and 300 mg/kg decreased significantly AST, at doses 200 and 300 mg/kg decreased ALT and only at dose 300 mg/kg decreased ALP serum levels. Administration of extract for 14 days at all doses decreased significantly ALP, at doses 200 and 300 mg/kg decreased ALT and only at dose 300 mg/kg decreased significantly AST serum levels (p<0.05)   Conclusion: O. persica. metanolic extract reduces the serum level of glucose in diabetic rats significantly. Based on our results, not only O. persica has no side effect regarding the function of liver, but also imporves this function.   Key words : Diabetes mellitus, Otostegia persica, Liver enzymes, Rat     Funding: This research was funded by Shahid Bahonar University of Kerman.   Conflict of interest : None declared.   Ethical approval : This study followed principles in the Declaration of Helsinki.     How to cite this article : Hedayati M, Pouraboli I, Mirtajaddini M. The Effect of Methanolic Extract of Otostegia persica on Serum Levels of Glucose and Liver Function Enzymes in Streptozotocin -Induced Diabetic Male Rats . J Rafsanjan Univ Med Sci 2011 10(2): 84-93. [Farsi]     [1] - MSc, Dept. of Biology, School of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran   [2] - Assistant Prof., Dept. of Biology, School of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran   Corresponding Autor, Tel: (0341) 3222032, Fax: (0341) 3222032, E-mail: pouraboli_i@mail. uk.ac.ir   [3] - MD, in Medicine, Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran